An S4 class to contain qPCR data.

The qPCR class is the main class of the qpcrtools package and is designed to contain all relevant qPCR data objects.

qPCR(dCT, factors, reference, metadata = NULL, genes = NULL)

Arguments

dCT	Imported dCT qPCR data as a data.table (i.e., output of read_dct()).
factors	A character vector listing factors for statistical comparison corresponding to a subset of the columns of each data.table object.
reference	A character vector of the same length as factors, listing the level of each factor corresponding to the reference group.
metadata	Columns in the dCT data.table which are not genes, but are not relevant for comparison. Either specify metadata or genes.
genes	A character vector listing genes corresponding to a subset of the columns of each data.table object.

Author

Ayush Noori

Examples

if (FALSE) qPCR(factors = c("Genotype", "Treatment"), reference = c("APOE3", "PBS"), metadata = c("Mouse", "Sex"))